Females were all same aged (3 days old) and either mated or not. Mated females were dissected at the following timeepoints: 0, 3, 6, 24 hrs postmating. Unmated (control) females were dissected at times interspersed with dissections of mated females.
Growth protocol
12 hr light/dark cycle 23C + or - 2C holding and culturing regimes
Extracted molecule
total RNA
Extraction protocol
TRIzol - chloroform
Label
Biotin (Enzo RNA transcript Labeling Kit)
Description
Three-day-old mated and unmated females were dissected to remove the lower reproductive tract (upper uterus, sperm-storage organs, and accessory glands). Mated females were dissected either immediately following mating (0 hr) or at 3, 6, or 24 hrs following the termination of mating. Tracts of 12-40 females of like category were pooled and total RNA extracted via a TRIzol-based protocol. Processing and labeling of transcript was performed by the Molecular Biology Core Facility at the Medical College of Georgia. Arrays from mated females at the different timepoints were compared to unmated females. This sample is also known as "217.1C.cel" and "CtrlUGA1C.CEL".
Data processing
RMA (Irizarry 2003), probe-based linear model (Chu et al 2002)
