array type: HT_Rat230_PM amount to core: 30 units of amount: ul od260: 3.389 od280: 1.694 dilution: 1:03 rna integrity number: 9.7 bioanalyzer results: Excellent sample preservation: RNA Later strain or line: F344/DuCrl ncbi taxonomy id: 10114 individual: 113 developmental state: Adult sacrifice method: Pentobarbital, Exsanguinated Sex: Female age: 10 units of age: Weeks tissue: Thyroid tissue harvest methods: The gland was transversely sectioned and the bottom portion was used for microarray analysis
Biomaterial provider
L. Pluta, The Hamner Institutes for Health Sciences, 6 Davis Drive, Research Triangle Park, NC, 27709, L. Pluta, [email protected], 919 558 1357
Treatment protocol
in vivo Treatment: 4,4’-Methylenebis(N,N-dimethyl)benzenamine, Treatment Repeat: 7, Units of Treatment Repeat: Days/Week, Treatment Duration: 2, Units of Duration: Weeks, Treatment Time: ad lib, Vehicle for Chemical: Rodent Chow, Route of Exposure: Diet, Dose or Concentration: 750, Units of Dose or Concentration: ppm
Extracted molecule
total RNA
Extraction protocol
Extraction Method: Qiagen
Label
biotin
Label protocol
standard Affymetrix protocol
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
LP_750_2w_113_MDMB750_10011_RThyroid_(HT_Rat230_PM)_E10: 1470 rats were used in this study: 6 chemicals, 4 time points, and 6 dose groups (including control). 10 animals per group and 5 extra control animals. Timepoints were 5d, 2w, 4w, and 13 weeks. Doses varied with compound (Tribromobenzene: 0, 2.5, 5, 10, 25, 75; Bromobenzene: 0, 25, 100, 200, 300, 400; Tetrachlorophenol: 0, 10, 25, 50, 100, 200; Methylenebis(N,N-dimethyl)benzen amine: 0, 50, 200, 375, 500, 750; Nitrosodiphenylamine: 0, 250, 1000, 2000, 3000, 4000; Hydrazobenzene: 0, 5, 20, 80, 200, 300). Animals were dosed through gavage or diet. Two of the protocols had female rats and the rest were male rats (see protocols). F344 and Sprague Dawley rats were used.
