array type: HT_Rat230_PM amount to core: 40 units of amount: ul od260: 8.912 od280: 4.334 ratio: 2.06 bioanalyzer results: Excellent sample preservation: Frozen strain or line: Spague Dawley Crl:CD(SD) ncbi taxonomy id: 10114 individual: 575 developmental state: Adult sacrifice method: Pentobarbital, Exsanguinated Sex: Male units of age: Weeks tissue: Liver tissue harvest methods: Slices from each of the lobes were pooled together for microarray analysis
Biomaterial provider
L. Pluta, The Hamner Institutes for Health Sciences, 6 Davis Drive, Research Triangle Park, NC, 27709, L. Pluta, [email protected], 9195581357
Treatment protocol
in vivo Treatment: 2,3,4,6-Tetrachlorophenol, Treatment Repeat: 7, Units of Treatment Repeat: Days/Week, Treatment Duration: 5, Units of Duration: Days, Treatment Time: 1, Units of Treatment Time: exposure/day, Vehicle for Chemical: Olive Oil, Amount of Vehicle: 5, Route of Exposure: Gavage, Units of Amount of Vehicle: ml/kg, Dose or Concentration: 200, Units of Dose or Concentration: mg/kg
Extracted molecule
total RNA
Extraction protocol
Extraction Method: Qiagen
Label
biotin
Label protocol
standard Affymetrix protocol
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
LP_200_5d_575_TTCP200_10010_Liver_(HT_Rat230_PM)_C06: 1470 rats were used in this study: 6 chemicals, 4 time points, and 6 dose groups (including control). 10 animals per group and 5 extra control animals. Timepoints were 5d, 2w, 4w, and 13 weeks. Doses varied with compound (Tribromobenzene: 0, 2.5, 5, 10, 25, 75; Bromobenzene: 0, 25, 100, 200, 300, 400; Tetrachlorophenol: 0, 10, 25, 50, 100, 200; Methylenebis(N,N-dimethyl)benzen amine: 0, 50, 200, 375, 500, 750; Nitrosodiphenylamine: 0, 250, 1000, 2000, 3000, 4000; Hydrazobenzene: 0, 5, 20, 80, 200, 300). Animals were dosed through gavage or diet. Two of the protocols had female rats and the rest were male rats (see protocols). F344 and Sprague Dawley rats were used.
