array type: HT_Rat230_PM amount to core: 50 units of amount: ul od260: 1.992 od280: 0.929 ratio: 2.07 rna integrity number: 8.8 bioanalyzer results: Good sample preservation: Frozen strain or line: Spague Dawley Crl:CD(SD) ncbi taxonomy id: 10114 individual: 163 developmental state: Adult sacrifice method: Pentobarbital, Exsanguinated Sex: Male age: 11 units of age: weeks tissue: Liver tissue harvest methods: Slices from each of the lobes were pooled together for microarray analysis
Biomaterial provider
L. Pluta, The Hamner Institutes for Health Sciences, 6 Davis Drive, Research Triangle Park, NC, 27709, L.Pluta, [email protected], 9195581357
Treatment protocol
in vivo Treatment: 1,2,4-Tribromobenzene, Treatment Repeat: 7, Units of Treatment Repeat: Days/Week, Treatment Duration: 4, Units of Duration: Weeks, Treatment Time: 1, Units of Treatment Time: exposure/day, Vehicle for Chemical: Corn Oil, Amount of Vehicle: 5, Route of Exposure: Gavage, Units of Amount of Vehicle: ml/kg, Dose or Concentration: 25, Units of Dose or Concentration: mg/kg
Extracted molecule
total RNA
Extraction protocol
Extraction Method: Qiagen
Label
biotin
Label protocol
standard Affymetrix protocol
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
LP_25_4w_163_TRBZ_10008_Liver_(HT_Rat230_PM)_G11: 1470 rats were used in this study: 6 chemicals, 4 timepoints, and 6 dose groups (including control). 10 animals per group and 5 extra control animals. Timepoints were 5d, 2w, 4w, and 13 weeks. Doses varied with compound (Tribromobenzene: 0, 2-5,5,10,25,75; Bromobenzene: 0, 25, 100, 200, 300, 400; Tetrachlorophenol: 0, 10, 25, 50, 100, 200; Mehtylenebis(N,N-dimethyl)benzenamine: 0, 50, 200, 375, 500, 750; Nitrosodiphenylamine: 0, 250, 1000, 2000, 3000, 4000; Hydrazobenzene: 0, 5, 20, 80, 200, 300). Animals were dosed through gavage or diet. Two of the protocols had female rats and the rest were male rats (see protocols). F344 and Sprague Dawley rats were used.
