|
| Status |
Public on Aug 16, 2013 |
| Title |
272_12 |
| Sample type |
RNA |
| |
|
| Source name |
Tibialis anterior muscle_Glycerol_3 days
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6J
gender: male
age: 12 weeks
genotype/variation: wild type
injected with: 25μl of 50% v/v glycerol
days after injection: 3 days
tissue: Tibialis anterior muscle
|
| Treatment protocol |
25μl of 50% v/v glycerol or 25μl of 10μM cardiotoxin was injected into tibialis anterior muscles of mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the snap-fozen tibialis anterior muscle using an RNeasy Fibrous Tissue Mini Kit (Qiagen), and eluted in 40μl RNase free water. RNA concentration and quality was measured by a ND-1000 spectrophotometer (NanoDrop, Thermo Scientific). In case RNA quality was not acceptable (ratio 260/230<1.5), samples were cleaned using a RNeasy Plus Micro Kit (Qiagen), eluted in 15 μl and the new concentration and ratios measured again. RNA final quality was assessed on an Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano kit, and processed for microarray when RIN>7
|
| Label |
FL cDNA Biotin Module V2
|
| Label protocol |
Nugen Ovation RNA Amplification System+ WB + FL cDNA Biotin Module V2
|
| |
|
| Hybridization protocol |
Affymetrix Hybridization control kit (including B2)
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
SAMPLE 11
|
| Data processing |
RMA normalization followed by 2% 150 trimmed mean scaling. Probesets with scaled RMA values below 50 in all groups were filtered out (R/BioC, simpleaffy_2.28.0).
|
| |
|
| Submission date |
Mar 28, 2013 |
| Last update date |
Aug 16, 2013 |
| Contact name |
Sophie Brachat |
| E-mail(s) |
[email protected]
|
| Organization name |
Novartis Institutes For Biomedical Research
|
| Department |
Muskuloskeletal
|
| Street address |
Novartis Campus
|
| City |
Basel |
| ZIP/Postal code |
CH-4002 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE45577 |
Profiling of glycerol- and CTX-induced models of muscle regeneration in mice |
|
