|
| Status |
Public on Mar 01, 2015 |
| Title |
AML-6P CD34-CD38- cells |
| Sample type |
RNA |
| |
|
| Source name |
childhood AML diagnostic BM
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: leukemia stem cell CD34-CD38-
chemo-resistance cell type: CD34+ > CD34-
chemo-resistance lc50 type: LC50medium
disease state: childhood AML
subject: AML-6P
|
| Treatment protocol |
none (the samples had been frozen in 10%DMSO/90%FCS)
|
| Growth protocol |
washed in RPMI with 10%FCS and Pen/strep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol/chloroform/RNAeasy (Qiagen) integrated method - amplified using NuGEN WTA Pico Ovation V2 kit
|
| Label |
biotin
|
| Label protocol |
Fragmented and Labelled with NuGEN Encore Biotin Module
|
| |
|
| Hybridization protocol |
Affymetrix Standard protocol
|
| Scan protocol |
Arrays were scanned with a GeneChip3000 7G scanner
|
| Description |
Gene expression data from Lin-CD34-CD38- leukaemia stem cells
|
| Data processing |
Probe level quantile normalisation and robust multi-array analysis on the raw CEL files were performed using the affy package of the Bioconductor project. Differentially expressed probe sets were identified using limma with fold change >1.5 and p<0.01.
|
| |
|
| Submission date |
Mar 18, 2013 |
| Last update date |
Mar 01, 2015 |
| Contact name |
Wenbin Wei |
| E-mail(s) |
[email protected]
|
| Organization name |
Durham University
|
| Department |
Biosciences
|
| Street address |
Stockton Road
|
| City |
Durham |
| ZIP/Postal code |
DH1 3LE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE45249 |
CD34 negative populations are associated with an increased relapse risk in childhood AML and harbor chemo-resistant leukemia stem cells |
|
