|
| Status |
Public on Apr 16, 2013 |
| Title |
KTaj-NSM-FCQTP_100-1a |
| Sample type |
RNA |
| |
|
| Source name |
Frontal cortex
|
| Organism |
Mus musculus |
| Characteristics |
region: Frontal cortex
gender: male
strain: CD-1
treatment: quetiapine
dose: 100
|
| Treatment protocol |
Male CD-1 mice were administered vehicle (0.5% methycellulose twice a day), quetiapine (10 or 100 mg/kg twice a day) or haloperidol (0.32 or 1 mg/kg twice a day) for 28 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frontal cortex and striatum were dissected and total RNA was extracted using an Rneasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
|
| |
|
| Hybridization protocol |
The hybridization cocktail was fragmented at 65oC for 30 min
|
| Scan protocol |
Scanning was performed according to the Affymetrix protocol at the Microarray Core Facility, Johns Hopkins University.
|
| Data processing |
Data analysis was performed using Partek Genomics Suite (version 6.5).
|
| |
|
| Submission date |
Mar 15, 2013 |
| Last update date |
Apr 16, 2013 |
| Contact name |
Jonathan Pevsner |
| E-mail(s) |
[email protected]
|
| Phone |
(443)923-2686
|
| Organization name |
Kennedy Krieger Institute
|
| Department |
Neurology
|
| Street address |
707 N. Broadway
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE45229 |
Unique pharmacological actions of atypical neuroleptic quetiapine: possible role in cell cycle/fate control |
|
