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| Status |
Public on Mar 14, 2014 |
| Title |
11c255-F-Dev5 [mRNA] |
| Sample type |
SRA |
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| Source name |
11c255-F-Dev5
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| Organism |
Capsicum annuum |
| Characteristics |
inbred line: 11c255
tissue: Fruits
developmental stage: mature green fruit
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| Extracted molecule |
total RNA |
| Extraction protocol |
Different tissues were removed respectively, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
Beads with oligo(dT) were used to isolate poly(A) mRNA after total RNA was collected from eukaryote . Fragmentation buffer was added for interrupting mRNA to short fragments. Taking these short fragments as templates, Random hexamer-primer were used to synthesize the first-strand cDNA. The second-strand cDNA was synthesized using buffer, dNTPs, RNase H and DNA polymerase I, respectively. Short fragments were purified with QiaQuick PCR extraction kit and resolved with EB buffer for end reparation and adding poly(A). After that, the short fragments were connected with sequencing adaptors. And, for amplification with PCR, we selected suitable fragments, as templates, with respect to the result of agarose gel electrophoresis. At last, the library could be sequencing using Illumina HiSeqTM 2000.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
C.annuum 11c255
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| Data processing |
Genome_build: Pepper Ref_v1.0 (ASJU00000000)
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| Submission date |
Mar 12, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
CNSA CNGB |
| Organization name |
BGI
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| Street address |
BGI
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| City |
shenzhen |
| ZIP/Postal code |
518083 |
| Country |
China |
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| Platform ID |
GPL16195 |
| Series (2) |
| GSE45037 |
Transcriptome sequencing analysis of pepper |
| GSE45154 |
Comparative genomics of domesticated peppers and its wild progenitor reveal signature of artificial selection by human |
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| Relations |
| SRA |
SRX248830 |
| BioSample |
SAMN01975460 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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