|
| Status |
Public on Dec 31, 2013 |
| Title |
Klf4 Morpholino injection replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Klf4 Morpholino injection
|
| Organism |
Danio rerio |
| Characteristics |
tissue: whole embryo
genotype/variation: wild type
hours post fertilization: 4.7
microinjection: Klf4 Morpholino
|
| Treatment protocol |
Embryos were injected with 4 ng/embryo of Klf4 Morpholino (MO), 8 ng/embryo of Standard Control MO, FoxD5 MO or FoxD3 MO, or 4ng/embryo of FoxD3MO+4ng/embryo of FoxD5MO. Embryos were frozen at 4,7 hpf for RNA isolation
|
| Growth protocol |
WT (wild type) were used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
60-100 embryos per sample were snap-frozen in liquid nitrogen, and total RNA was isolated using the RNA Easy kit (Qiagen). Sample quality was assessed in an Agilent Bioanalyzer 2100, using the RNA 6000 nano Assay Kit
|
| Label |
Cy3
|
| Label protocol |
Samples were processed by Agilent Technologies Two-Color Microarray-Based Gene Expression Analysis kit according to Agilent Two-color Microarray based gene expression analysis protocoll
|
| |
|
| Hybridization protocol |
Agilent Gene Expression Hybridization Kit was used . Micorarrays were hybridized in the Agilent 2545A hybridization oven according Agilent Two-color Microarray based gene expression analysis protocoll
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner
|
| Description |
US90900275_252643710068_S01_GE2_107_Sep09_QC_1_1.txt: Cy3
|
| Data processing |
Data were extracted using Agilent Feature Extraction Software and GE2_107_Sep09_QC protocol.
Further Normalisation and processing of the data was done using Genedata Expressionist and Analyst software. Cy3 and Cy5 channels of each microarray were normalized using quantile normalization
|
| |
|
| Submission date |
Mar 11, 2013 |
| Last update date |
Feb 06, 2023 |
| Contact name |
Daria Onichtchouk |
| E-mail(s) |
[email protected]
|
| Phone |
00497612032595
|
| Organization name |
University of Freiburg
|
| Street address |
Hauptstrasse 1
|
| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14664 |
| Series (2) |
| GSE45012 |
Experiment 4: Klf4, FoxD3, FoxD5 and combined FoxD3 and FoxD5 loss-of-function experiments at 30% epiboly Wild Type zebrafish embryos |
| GSE45013 |
Transcriptional targets of Pou5f1 in Zebrafish (Danio Rerio) |
|
