|
| Status |
Public on May 20, 2006 |
| Title |
KINECells_Chemokinetic |
| Sample type |
RNA |
| |
|
| Source name |
H460_Selected_For_Chemokinesis
|
| Organism |
Homo sapiens |
| Characteristics |
H460 Lung Cancer Cells Selected for highly chemokinetic trait.
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
Cells were selected for behaviour under chemokinetic conditions (no gradient of chemoattractant [1.5%FBS]) using Boyden chambers.
|
| Growth protocol |
Cells grown were in complete medium (DMEM + 5% FBS). No antibiotics were used for the experiment. Cells were cultured for 24 h prior to harvesting for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard RNA extraction procedure using RNAeasy Mini kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
cRNA was labeled with biotin using the Affymetrix Enzo Kit according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Hybridization, washing and staining were performed in a Fluidics Station according to standard procedures. Briefly, 15 micrograms of biotinylated cRNA were hybridized to a U133A chip for 16 h, and labeld with anti-streptavidin biotinylated antibody with intermittent washing steps.
|
| Scan protocol |
Affymetrix® GeneChip® Scanner3000 was used to scan the chip with preset pixel resolution and wavelength values.
|
| Description |
N/A
|
| Data processing |
Affymetrix MAS 5.0 Scaling Protocol. Original signal values are scaled to a mean of 1000.
|
| |
|
| Submission date |
May 18, 2006 |
| Last update date |
May 19, 2006 |
| Contact name |
Lilian Soon |
| E-mail(s) |
[email protected]
|
| Phone |
293515322
|
| Fax |
2 9351 7682
|
| Organization name |
The University of Sydney
|
| Department |
Australian Key Centre for Microscopy and Microanalysis, EMU
|
| Lab |
Cancer Cell Imaging
|
| Street address |
LG14, Madsen Building, F09
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE4869 |
Chemokinetic versus Chemotactic Cancer Cells |
|
