|
| Status |
Public on May 18, 2007 |
| Title |
Cochlea_WT_9mo_5 |
| Sample type |
RNA |
| |
|
| Source name |
RNA
|
| Organism |
Mus musculus |
| Characteristics |
Strain: Polg+/+ mice
Gender: male
Age: 9 month-old
Tissue: cochlea
For additional details about the strain used please refer to the following reference:
Kujoth, G.C. et al. (2005) Mitochondrial DNA mutations, oxidative stress, and apoptosis in mammalian aging. Science 309, 481-4.
|
| Biomaterial provider |
created by our laboratory
|
| Treatment protocol |
To identify and analyze mtDNA mutation-responsive genes, a comparison of cochlea tissues from WT (control) and D257A mice was conducted. We examined changes in gene expression in the cochleae induced by age-related accumulation of mtDNA mutations.
|
| Growth protocol |
All mice were raised under 12:12 dark/light conditions, and housed in the specific pathogen-free facility at University of Wisconsin-Madison, and provided a nonpurified diet and acidified water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cochlea tissues using the TriZol Reagent (Invitrogen) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
cDNA synthesis from total RNA (Invitrogen), cRNA amplification and labeling (Enzo BioArray IVT kit) were accomplished according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Hybridization (hybridization oven 640) (Affymetrix), and washing and staining (fluidics station 400) (Affymetrix) were accomplished according to the manufacturer’s instructions.
|
| Scan protocol |
Arrays were scanned on the GeneChip Scanner 3000 (Affymetrix).
|
| Description |
NA
|
| Data processing |
GCOS 1.3 (Affymetrix), scaled to 1500
|
| |
|
| Submission date |
May 18, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shinichi Someya |
| E-mail(s) |
[email protected]
|
| Phone |
608-265-5205
|
| Fax |
608-262-2976
|
| URL |
http://www.genetics.wisc.edu/faculty/profile.php?id=140
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Medical Genetics
|
| Lab |
Prolla Lab
|
| Street address |
425 Henry Mall Dr
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE4866 |
The role of mtDNA mutations in age-related hearing loss in mice carrying a mutator DNA polymerase gamma |
|
| Relations |
| Reanalyzed by |
GSE119085 |
