|
| Status |
Public on Mar 05, 2013 |
| Title |
CLL71_C |
| Sample type |
RNA |
| |
|
| Source name |
Human CLL cells
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: CLL
agent: control
cell type: B-cells purified using negative selection
|
| Treatment protocol |
MLN4924 1 microM
|
| Growth protocol |
Cells were cultured in RPMI with 15% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
B-cells isolated using B-cell purification kit (Multenyi Biotech). RNA isolated using RNEasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Reverse transcription using an oligo(dT) primer bearing a T7 promoter and the high yield ArrayScriptTM reverse transcriptase were used to make cDNA. The cDNA was made double-stranded and purified to use as a template for in vitro transcription with T7 RNA Polymerase and the included biotin-NTP mix
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina hybridization protocol
|
| Description |
SAMPLE 1
CLL cells from patient with CLL
|
| Data processing |
The data were normalized using quantile normalisation with BRB Array Tools
|
| |
|
| Submission date |
Mar 05, 2013 |
| Last update date |
Mar 05, 2013 |
| Contact name |
Alexey Danilov |
| Organization name |
Oregon Health and Science Univeristy
|
| Street address |
3181 SW Sam Jackson Pk Rd
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97219 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE44864 |
MLN4924 in chronic lymphocytic leukemia |
|
