|
| Status |
Public on May 12, 2006 |
| Title |
32B |
| Sample type |
RNA |
| |
|
| Source name |
SMAA/EYFP postnatal day 1 bladder
|
| Organism |
Mus musculus |
| Characteristics |
gene reported: SMAA/EYFP transgenic / PMID: 14713859
strain: FVB/N
sex: Male
developmental stage: postnatal day 1
theiler stage: 27
somite count: NA
developmental landmark: NA
|
| Treatment protocol |
pool size: N/A
Pooled sample: no
Dissection Method: whole bladder
FVB/N female mice are time-mated to SMGA/EGFP or SMAA/EYFP transgenic males. Pregnant FVB/N mice are euthanized by standard carbon dioxide asphyxiation. All fetuses and newborn mice are killed by decapitation with a scalpel. Tissues are harvested and used whole or placed in OCT in a mold, frozen and stored at -80 °C. Blocks are sectioned, stained and used for laser capture micro-dissection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen: RNeasy
|
| Label |
Biotin
|
| Label protocol |
-
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
Amount labeled target hybridization to array: 10 ug
|
| Scan protocol |
-
|
| Description |
>> Amplification protocol <<
Target Amplified manufacturer/kit: Epicentre
Target Amplified protocol: Potter protocols
Rounds of amplification: 1
URL: http://gudmap.hgu.mrc.ac.uk/gudmap/pages/mic_submission.html?id=GUDMAP:2197
|
| Data processing |
Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
|
| |
|
| Submission date |
May 12, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
[email protected]
|
| Phone |
+44 131 651 8500
|
| Organization name |
IGMM MRC Human Genetics Unit
|
| Lab |
GUDMAP Database Group
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE4816 |
Gene expression profiles of the P1 bladder isolated from SMAA or SMAGA (Acta2 or Actg2) transgenic and wild type mice. (GUDMAP Series ID: 2) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
