|
| Status |
Public on May 03, 2007 |
| Title |
Gastrin infused gastrin Knockout replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Gastrin infused gastrin Knockout fundus
|
| Organism |
Mus musculus |
| Characteristics |
Gastrin Knockout infused with gastrin, 2 month old male mice fundic mucosal scraping
|
| Extracted molecule |
total RNA |
| Extraction protocol |
gastrin knockout mice were infused with gastrin for 1 week and the fundus was used to isolate RNA from fundic mucosal scrapings. Total RNA was extracted by Trizol followed by an RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Approximately 1 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Gastrin Knockout infused with gastrin fundic RNA
|
| Data processing |
Probe-set signal intensity from Ann Arbor Suite. See http://dot.ped.med.umich.edu:2000/ourimage/pub/shared/Affymethods.html or Affymethods.pdf supplementary file associated with GSE4810.
|
| |
|
| Submission date |
May 11, 2006 |
| Last update date |
May 03, 2007 |
| Contact name |
Linda Samuelson |
| E-mail(s) |
[email protected]
|
| Phone |
734-764-9448
|
| Fax |
734-936-8813
|
| Organization name |
University of Michigan
|
| Department |
Molecular and Integrative Physiology
|
| Street address |
MED SCI II, # 7761, 1150 W. MEDICAL CENTER DRIVE
|
| City |
ANN ARBOR |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE4810 |
Fundic mucosa gene expression in the presence/absence of gastrin and in gastrin-CCK double knockout mice. |
|
