|
| Status |
Public on May 06, 2014 |
| Title |
CD4+ T cells, stimulated - rep4 |
| Sample type |
RNA |
| |
|
| Source name |
from PBMCs, 30 h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Immune cells
cell type: CD4+ T cells
treatment: stimulated
|
| Treatment protocol |
CD4+ T cells of each approach were pooled, counted and resuspended in TRIzol reagent.
|
| Growth protocol |
CD4+ T cells were prepared from 2 male and 2 female donors, enriched by negative selection (BD IMag) and cultured at 37°C, 5% CO2 for 30 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by chloroform/isopropanol extraction. For RNA concentration and purification the RNeasy MinElute Cleanup Kit (Qiagen) was used as recommended by the manufacturer.
|
| Label |
biotin
|
| Label protocol |
Reverse transcription of RNA to synthesize cDNA was done by using the WT Expression Kit (Ambion). cDNA fragmentation and labeling was performed by applying the GeneChip WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Probes were prepared using the GeneChip Hybridization, Wash, and Stain Kit (Affymetrix) and analyzed by microarray technology (Affymetrix GeneChip Human Gene 1.0 ST Array).
|
| Scan protocol |
The gene-expression scan was performed with the GeneChip Command Console 3.0 Software (Affymetrix).
|
| Description |
Donor4_stim
Gene expression data from CD4+ T cells
|
| Data processing |
The robust multi-array average (RMA) algorithm was applied for background correction, using the Expression Console 1.1 Software (Affymetrix). The samples were quantile normalized, followed by mean signal summarization.
The samples were quantile normalized, followed by mean signal summarization. Hierarchical clustering, t-test and significance analysis of microarrays (SAM) were performed using the TIGR multi experiment viewer (MeV) 4.6.1 Software (TM4 Development Group).
|
| |
|
| Submission date |
Feb 19, 2013 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Thomas Streichert |
| Organization name |
University Medical Center Hamburg-Eppendorf
|
| Department |
Institute For Clinical Chemistry / Central Laboratories
|
| Street address |
Martinistr. 52, Campus Forschung N27, 2. OG
|
| City |
Hamburg |
| ZIP/Postal code |
D-20246 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE44392 |
Expression data from stimulated or unstimulated human CD4+ T cells incubated with edelfosine |
|
| Relations |
| Reanalyzed by |
GSE86357 |
