|
| Status |
Public on Oct 09, 2013 |
| Title |
In vitro EPS-stimulated myotubes, biological replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
In vitro EPS-stimulated myotubes
|
| Organism |
Homo sapiens |
| Characteristics |
individual id: individual 05
age (yrs): 19
gender: male
tissue: primary human myotubes
sample group: in-vitro electric pulse stimulation (EPS) for 24hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen) including DNase treatment
|
| Label |
biotin
|
| Label protocol |
WT Expression Kit (Ambion) and the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol
|
| Scan protocol |
According to the Affymetrix expression protocol
|
| Description |
MT05_stim
|
| Data processing |
Expression console (Build 1.2.1.20, Affymetrix) was used to obtain normalized RMA gene-level data (standard settings including sketch-quantile normalisation)
|
| |
|
| Submission date |
Feb 04, 2013 |
| Last update date |
Oct 09, 2013 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
[email protected]
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE44051 |
Cytokines dominate the secretome of primary human myotubes in an in vitro exercise model |
|
