|
| Status |
Public on Feb 20, 2013 |
| Title |
LSK+CD34- HSCs from 2 mo old female Xist wild-type (2lox/+) |
| Sample type |
RNA |
| |
|
| Source name |
FACS sorted LKS+CD34- hematopoietic stem cells (HSC)
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Xist wild-type (2lox/+) mouse
age: 2 months old
gender: female
genetic background: 129Sv/Jae
cell type: LKS+CD34- hematopoietic stem cells (HSC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from cells was isolated using Trizol reagent (Invitrogen).
|
| Label |
Biotin
|
| Label protocol |
RNA was labeled using NuGen Ovation Pico WTA v2 System paired with the Encore Biotin Module (NuGEN).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5ug of cDNA were hybridized for 16 hrs at 45C on Affymetrix Mouse Gene 1.0ST microarrays. GeneChps were washed and stained in the Affymetric Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix Genechip Scanner 3000 7G. The data was collected using the Command Console Version 3.2.
|
| Description |
Gene expression data from LSK+CD34- HSCs from 2 months old female Xist wild-type (2lox/+) mouse.
Hematopoietic cells were isolated from bone or spleen of mouse, indicated cell lineages were FACS sorted and used to isolate RNA as described under Methods in Yildirim et al. Cell 2013.
|
| Data processing |
The data were analyzed using R Bioconductor Affy package, with RMA applied as summarization and normalization method (Irizarry et al., 2003).
|
| |
|
| Submission date |
Jan 31, 2013 |
| Last update date |
Jul 22, 2022 |
| Contact name |
Ruslan Sadreyev |
| E-mail(s) |
[email protected]
|
| Phone |
6176435697
|
| Organization name |
Harvard Medical School/ Mass General Hospital
|
| Street address |
185 Cambridge St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE43961 |
Xist RNA is a potent suppressor of hematologic cancer in mice. |
|
