|
| Status |
Public on Jul 03, 2007 |
| Title |
Arabidopsis_Chitooctaose-30M_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis 2-week-old seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Ecotype: Col 0
|
| Treatment protocol |
2-week-old seedlings were treated with 20 ul 0.5 mM chitooctaose (with a final concentration of 1 uM) for 30 minutes, with gentle shaking.
|
| Growth protocol |
Seedlings (~ 30 seeds at the beginning) were grown hydroponically in a 50-ml falcon tube containing 10 ml of liquid medium (1X Murashige and skoog basal salt mixture + 2% dextrose, pH 5.8). The tube was incubated with gentle shaking in a growth chamber with the following settings: constant light at 125 umol m-2 s-1 at a temperature of 23 C. After 14 days, 20 ul of chitooctaose was added to the mdieum containing the seedlings to achieve a final concentration of 1 uM. Whole seedlings were collected 30 minutes after treatment, immediately frozen in liquid nitrogen and stored at -80 C for later use..
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Arabidopsis Genome Array ATH1. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from 14-day-old Arabidopsis seedlings treated with water.
|
| Data processing |
The data were normalized and analyzed with DNA-Chip Analyzer (dChip; Version Release, April 1, 2005) using the default settings.
|
| |
|
| Submission date |
May 01, 2006 |
| Last update date |
Jul 03, 2007 |
| Contact name |
Gary Stacey |
| E-mail(s) |
[email protected]
|
| Phone |
573-884-4799
|
| URL |
http://psu.missouri.edu/staceylab/index.htm
|
| Organization name |
University of Missouri-Columbia
|
| Department |
Plant Sciences
|
| Street address |
1201 Rollins Road
|
| City |
Columbia |
| State/province |
MO |
| ZIP/Postal code |
65211 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE4746 |
Expression data from Arabidopsis 14-day-old seedlings |
|
