|
| Status |
Public on May 08, 2006 |
| Title |
Rat_Ventricle_Diabetic_42days_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Diabetic Rat ventricle after 42 days, diastolic and systolic dysfunction
|
| Organism |
Rattus norvegicus |
| Characteristics |
Genotype: male Wistar rat 150g at time of injection
State: Diabetic
Age: 42 days post injection
Body Weight (g): 126
Ventricle Weight (g): 0.40
Blood Glucose (mg/dL): 582
Ventricle Weight/Body Weight Ratio: 3.175
Body Weight vs Control average: 0.291
|
| Treatment protocol |
150g male Wistar rats (Harlan) were injected with 65 mg/kg streptozotocin (STZ) (Diabetic) or citrate buffer (Control). Ventricles were collected and frozen after 3/28/42 days, corresponding to diabetes response with no symptoms, diastolic symptoms, and full diastolic and systolic symptoms of diabetic cardiomyopathy.
|
| Growth protocol |
Rats were kept separate in steel cages with full access to standard feed and water. Ventricles removed at 03/28/42 day time points, frozen and total RNA extracted by Trizol method
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized on Affymetrix GeneChip Rat genome RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
|
| Description |
Gene expression data from diabetic rat ventricles 42 days after injection
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 01, 2006 |
| Last update date |
May 04, 2006 |
| Contact name |
Mohammed Abdul Matlib |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Cincinnati
|
| Department |
Pharmacology and Cell Biophysics
|
| Street address |
PO Box 670575
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45267-0575 |
| Country |
USA |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE4745 |
Expression data from Rat ventricles 3 days/28 days/42 days after STZ injection |
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