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Sample GSM107422 Query DataSets for GSM107422
Status Public on May 08, 2006
Title Rat_Ventricle_Control_28days_rep3
Sample type RNA
 
Source name Control Rat ventricle after 28 days
Organism Rattus norvegicus
Characteristics Genotype: male Wistar rat 150g at time of injection
State: Control
Age: 28 days post injection
Body Weight (g): 312
Ventricle Weight (g): 0.71
Blood Glucose (mg/dL): 103
Ventricle Weight/Body Weight Ratio: 2.276
Body Weight vs Control average: 0.969
Treatment protocol 150g male Wistar rats (Harlan) were injected with 65 mg/kg streptozotocin (STZ) (Diabetic) or citrate buffer (Control). Ventricles were collected and frozen after 3/28/42 days, corresponding to diabetes response with no symptoms, diastolic symptoms, and full diastolic and systolic symptoms of diabetic cardiomyopathy.
Growth protocol Rats were kept separate in steel cages with full access to standard feed and water. Ventricles removed at 03/28/42 day time points, frozen and total RNA extracted by Trizol method
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized on Affymetrix GeneChip Rat genome RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
Description Gene expression data from control rat ventricles 28 days after injection
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 01, 2006
Last update date May 04, 2006
Contact name Mohammed Abdul Matlib
E-mail(s) [email protected]
Organization name University of Cincinnati
Department Pharmacology and Cell Biophysics
Street address PO Box 670575
City Cincinnati
State/province OH
ZIP/Postal code 45267-0575
Country USA
 
Platform ID GPL85
Series (1)
GSE4745 Expression data from Rat ventricles 3 days/28 days/42 days after STZ injection

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
A01157cds_s_at 121.4 A 0.32083
A03913cds_s_at 728.9 P 0.00418
A04674cds_s_at 160.6 A 0.64131
A07543cds_s_at 409.7 A 0.250724
A09811cds_s_at 64.4 A 0.933135
A16585cds_s_at 125.9 A 0.267463
A17753cds_s_at 27.2 A 0.781017
A30543cds_s_at 229.2 A 0.302547
A44407cds_at 22.3 A 0.951005
AA108277_at 243.3 A 0.098054
AA108308_i_at 6.5 A 0.982268
AA108308_s_at 1710.4 A 0.218983
AA684537_at 6245.2 P 0.003585
AA684929_f_at 1309.9 M 0.043968
AA684960_f_at 1378.9 A 0.11716
AA684963_at 1047.9 P 0.017001
AA685112_at 4822.5 P 0.000322
AA685152_r_at 32.6 A 0.870361
AA685376_f_at 662.9 A 0.339558
AA685876_at 1003.7 A 0.339558

Total number of rows: 8799

Table truncated, full table size 258 Kbytes.




Supplementary file Size Download File type/resource
GSM107422.CEL.gz 1.9 Mb (ftp)(http) CEL
GSM107422.EXP.gz 496 b (ftp)(http) EXP

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