|
| Status |
Public on Jan 26, 2013 |
| Title |
ES.2 - proliferating-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
ES.2 - proliferating
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Exponentially proliferating ovarian cancer cells
cell line: ES.2
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Ovarian cancer cell lines were plated and harvested when 50-80% confluent. RNA was extracted and hybridized on Affymetrix microarrays. At least 3 biological repeats were performed for each cell line.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAgen Rneasy was used to extract RNA
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
|
| |
|
| Hybridization protocol |
Hybridization was for 16hrs andaccording to the standard Affymetrix protocol
|
| Scan protocol |
GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450 using standard Fluidics Protocol ((Fluidics protocol FS450_007)). GeneChips were scanned using the Affymetrix 7G Scanner
|
| Data processing |
The data was gc-rma normalised using the R Bioconductor package affy.
|
| |
|
| Submission date |
Jan 25, 2013 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Karen Elizabeth Sheppard |
| E-mail(s) |
[email protected]
|
| Phone |
61 3 9656 3758
|
| Fax |
61 3 96563738
|
| Organization name |
Perter MacCallum Cancer Centre
|
| Department |
Research
|
| Street address |
St Andrews Place
|
| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3008 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE43765 |
Expression data from exponentially proliferating ovarian cancer cell lines |
|
| Relations |
| Reanalyzed by |
GSE86357 |
