|
| Status |
Public on May 24, 2013 |
| Title |
Monosome RNA DMSO-3 |
| Sample type |
other |
| |
|
| Source name |
Monosome fraction from DMSO treated parasites
|
| Organism |
Toxoplasma gondii |
| Characteristics |
strain: RH
rna population: Monosomal RNA
|
| Treatment protocol |
Purified parasites were treated with 10 uM tunicamycin or DMSO for 1 hour
|
| Growth protocol |
extracellular RH tachyzoites were purified from human fibroblast by filtration
|
| Extracted molecule |
other |
| Extraction protocol |
Total RNA or RNA from sucrose fractions were ethanol precipitated and purified using Rneasy columns
|
| Label |
Biotin
|
| Label protocol |
total RNA was labeled using the 3' IVT express kit from Affymetrix.
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol, except hybridization for 17 hours.
|
| Scan protocol |
Scanned using 3000-7G controlled by Affymetrix GCOS.
|
| Description |
Gene expression data from monosome fraction of control sample
|
| Data processing |
Affymetrix MAS5 data scaled to intensity of 1000.
|
| |
|
| Submission date |
Jan 24, 2013 |
| Last update date |
May 24, 2013 |
| Contact name |
Ron Wek |
| Organization name |
Indiana University School of Medicine
|
| Department |
Biochemistry & Molecular Biology
|
| Street address |
635 Barnhill Dr. MS4065
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platform ID |
GPL16542 |
| Series (1) |
| GSE43722 |
The Toxoplasma unfolded protein response |
|
