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Sample GSM1069686 Query DataSets for GSM1069686
Status Public on May 24, 2013
Title Total RNA DMSO-1
Sample type RNA
 
Source name Total RNA from DMSO treated parasites
Organism Toxoplasma gondii
Characteristics strain: RH
rna population: Total RNA
Treatment protocol Purified parasites were treated with 10 uM tunicamycin or DMSO for 1 hour
Growth protocol extracellular RH tachyzoites were purified from human fibroblast by filtration
Extracted molecule total RNA
Extraction protocol Total RNA or RNA from sucrose fractions were ethanol precipitated and purified using Rneasy columns
Label Biotin
Label protocol total RNA was labeled using the 3' IVT express kit from Affymetrix.
 
Hybridization protocol Affymetrix standard protocol, except hybridization for 17 hours.
Scan protocol Scanned using 3000-7G controlled by Affymetrix GCOS.
Description Gene expression data from control sample
Data processing Affymetrix MAS5 data scaled to intensity of 1000.
 
Submission date Jan 24, 2013
Last update date May 24, 2013
Contact name Ron Wek
Organization name Indiana University School of Medicine
Department Biochemistry & Molecular Biology
Street address 635 Barnhill Dr. MS4065
City Indianapolis
State/province IN
ZIP/Postal code 46202
Country USA
 
Platform ID GPL16542
Series (1)
GSE43722 The Toxoplasma unfolded protein response

Data table header descriptions
ID_REF
VALUE MAS5 signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1280.12 P 4.42873e-05
AFFX-BioB-M_at 1907.16 P 4.42873e-05
AFFX-BioB-3_at 1104.77 P 4.42873e-05
AFFX-BioC-5_at 3229.91 P 4.42873e-05
AFFX-BioC-3_at 2867.48 P 4.42873e-05
AFFX-BioDn-5_at 9219.94 P 4.42873e-05
AFFX-BioDn-3_at 11923 P 4.42873e-05
AFFX-CreX-5_at 41051.7 P 5.16732e-05
AFFX-CreX-3_at 42534.7 P 4.42873e-05
AFFX-DapX-5_at 528.105 P 0.000661624
AFFX-DapX-M_at 1153.91 P 4.42873e-05
AFFX-DapX-3_at 3022.75 P 5.16732e-05
AFFX-LysX-5_at 35.3373 A 0.48511
AFFX-LysX-M_at 30.3587 A 0.544595
AFFX-LysX-3_at 248.22 P 0.0151753
AFFX-PheX-5_at 174.191 P 0.00618711
AFFX-PheX-M_at 190.444 P 0.0396608
AFFX-PheX-3_at 285.285 P 0.0020226
AFFX-ThrX-5_at 7.79354 A 0.772383
AFFX-ThrX-M_at 165.951 P 0.0151753

Total number of rows: 8993

Table truncated, full table size 291 Kbytes.




Supplementary file Size Download File type/resource
GSM1069686_RWO5T001.CEL.gz 911.6 Kb (ftp)(http) CEL
GSM1069686_RWO5T001.CHP.gz 52.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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