|
| Status |
Public on Jan 19, 2014 |
| Title |
Irs2-/- treated-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
pancreatic islets Irs2-/- treated
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57Bl/6
genotype/variation: Irs2 knockout (Irs2 -/-)
age (at the end of treatment): 13 wks
gender: male
drinking water: distilled water containing 2mg/dl of sodium tungstate for 21 days
tissue: pancreatic islets
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized, washed and stained according to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program 3.0.0.1214, scanner GCS 3000-7G)
|
| Description |
14.CEL
|
| Data processing |
Raw data was background adjusted, normalized and summarized by Robust Multi-array Analysis (RMA) using the Affy package from Bioconductor (v.2.0.1) on R language (v.2.7.0).
|
| |
|
| Submission date |
Jan 18, 2013 |
| Last update date |
Jan 19, 2014 |
| Contact name |
Joana Oliveira |
| E-mail(s) |
[email protected]
|
| Organization name |
Idibaps
|
| Street address |
Rosselló
|
| City |
BArcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE43620 |
Effects of sodium tungstate administration in Irs2 -/- mice |
|
