|
| Status |
Public on Mar 01, 2013 |
| Title |
siIMP1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
control JAR cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: JAR
cell type: human choriocarcinoma cell line
transfected with: universal negtive control siRNA
|
| Treatment protocol |
siRNA was transfected into cells by Lipofectamine RNAi MAX following manufacturer's instructions
|
| Growth protocol |
JAR cells were maintained in RPMI1640 with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
0.5 g of total RNA was amplified by a low RNA input fluor linear amp kit and labeled with Cy3 (contol) or Cy5 (IMP1 siRNA-treated).
|
| |
|
| Channel 2 |
| Source name |
siIMP1 transfected JAR cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: JAR
cell type: human choriocarcinoma cell line
transfected with: siRNA targeting IMP1
|
| Treatment protocol |
siRNA was transfected into cells by Lipofectamine RNAi MAX following manufacturer's instructions
|
| Growth protocol |
JAR cells were maintained in RPMI1640 with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
0.5 g of total RNA was amplified by a low RNA input fluor linear amp kit and labeled with Cy3 (contol) or Cy5 (IMP1 siRNA-treated).
|
| |
|
| |
| Hybridization protocol |
The fragmented labeled cRNA was then pooled and hybridized to Agilent human whole genome oligo microarray 4 x 44K (Agilent Technologies) at 60°C for 17 h.
|
| Scan protocol |
With Agilent microarray scanner (Agilent Technologies) at 535 nm for Cy3 and 625 nm for Cy5.
|
| Description |
siIMP1
|
| Data processing |
Agilent Feature Extraction software (version 9.3; Agilent Technologies) was used to quantify signal and background intensity for each feature and substantially normalized the data by rank consistency linear LOWESS normalization method.
Fold ratios were obtained by the comparison of normalized data between groups. Only genes that were up- or down-regulated greater than 2-fold between the groups were filtered [*regulated_genes.txt available on Series records]
|
| |
|
| Submission date |
Jan 18, 2013 |
| Last update date |
Mar 01, 2013 |
| Contact name |
yeunting hsieh |
| Organization name |
TCVGH
|
| Department |
GYN
|
| Lab |
M.B.
|
| Street address |
Chugong Road Section3, 160
|
| City |
Taichung |
| ZIP/Postal code |
40705 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE43609 |
JAR cells: Control vs. siIMP1 transfected |
|
