|
| Status |
Public on May 15, 2006 |
| Title |
Polysome fraction from adult mouse testes, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
adult mouse, strain CD1
|
| Organism |
Mus musculus |
| Characteristics |
Polysome fraction from adult mouse testes, biological rep3, adult mouse, strain CD1 !Sample_characteristics_ch1=,
|
| Treatment protocol |
cytoplasmic extract of mouse testes were subjected to 10-30% sucrose gradient centrifugation.Total RNAs were extracted from fractions 11-17 from total 17 as polysome-associated fraction by Trizol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip MOE 430A Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GCS3000 scanner.
|
| Description |
Polysome fraction from adult mouse testes, biological rep3
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 24, 2006 |
| Last update date |
Apr 24, 2006 |
| Contact name |
Norman B Hecht |
| Organization name |
University of Pennsylvania
|
| Street address |
421 Curie Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE4711 |
Developmental changes in RNP and Polysome associated mRNAs in Mouse testes |
|
