|
Status |
Public on Apr 25, 2006 |
Title |
fibroblast_5azacontrol_rep2 |
Sample type |
RNA |
|
|
Source name |
primary dermal fibroblasts untreated
|
Organism |
Mus musculus |
Characteristics |
strain: B6; gender: mix; age: 0-2d; tissue: dermis; passage #: 4; treatment: control; biological replicate: 2
|
Treatment protocol |
untreated
|
Growth protocol |
Cultures were maintained in Dulbecco’s modified Eagle’s medium (DMEM;Invitrogen; Carlsbad, CA, USA) with L-glutamine and high glucose plus 10% FBS (Invitrogen) and 100U/mL penicillin streptomycin (Invitrogen) at 37oC and 5% CO 2. Cells were passaged at confluence by 0.25% trypsin EDTA (Invitrogen).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy total RNA extraction protocol (Qiagen)
|
Label |
biotin labeled with standard Affymetrix fluorescent stain cocktail
|
Label protocol |
standard Affymetrix IVT labeling and staining protocols
|
|
|
Hybridization protocol |
standard Affymetrix hybridization protocol
|
Scan protocol |
standard Affymetrix scan protocol
|
Description |
untreated control; biological replicate 2
|
Data processing |
MAS 5.0 data processing and normalization
|
|
|
Submission date |
Apr 20, 2006 |
Last update date |
Apr 24, 2006 |
Contact name |
Tammy Vallender |
E-mail(s) |
[email protected]
|
Phone |
7738344065
|
Organization name |
University of Chicago
|
Department |
Human Genetics
|
Lab |
Dr. Bruce Lahn
|
Street address |
929 E 57th St W503V
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60637 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE4696 |
Gene expression changes following demethylation by 5-aza-2'-deoxycytidine in primary mouse dermal fibroblasts |
GSE4768 |
Global analysis of transcriptional changes following genomic demethylation in chondrocytes, osteoblasts, and fibroblasts |
|