|
| Status |
Public on Dec 29, 2012 |
| Title |
PBMC_Severe_Disease 3 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC, severe disease, mock
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: peripheral blood mononuclear cells (PBMCs)
treatment: mock
age: 60
gender: Male
batch: 1
disease status: Severe Disease
disease severity/outcome: Encephalitis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from PBMC samples was extracted using the RNeasy mini kit (Qiagen, CA)
|
| Label |
biotin
|
| Label protocol |
Preparation of cDNA, cRNA, and labeling were carried out by the Yale Center for Genomic Analysis using the standard Illumina protocols. Hybridization buffer from the BeadChip kit (Illumina) was mixed with 1500 ng of biotin-labeled cRNA, heated to 65°C for 5 minutes, and then loaded onto the BeadChip. The BeadChips were sealed in a hybridization chamber and placed in an oven at 58°C with a rocker for 16-20 hours. After the hybridization, the BeadChips were washed and stained in a series of washes and stains as outlined in the Illumina protocol. The BeadChips were scanned on the Illumina Iscan.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Severe Disease
|
| Data processing |
The data were normalised using quantile normalisation with lumi (lumi_2.4.0) in R version 2.13.2 (2011-09-30)
|
| |
|
| Submission date |
Dec 28, 2012 |
| Last update date |
May 06, 2013 |
| Contact name |
Hailong Meng |
| Organization name |
Yale University
|
| Street address |
300 George Str
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06525 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE43190 |
Serum predictors of susceptibility to infection with West Nile virus |
| GSE46681 |
Profiling the successful response to infection with West Nile virus |
|
