Total RNA from PBMC samples was extracted using the RNeasy mini kit (Qiagen, CA)
Label
biotin
Label protocol
Preparation of cDNA, cRNA, and labeling were carried out by the Yale Center for Genomic Analysis using the standard Illumina protocols. Hybridization buffer from the BeadChip kit (Illumina) was mixed with 1500 ng of biotin-labeled cRNA, heated to 65°C for 5 minutes, and then loaded onto the BeadChip. The BeadChips were sealed in a hybridization chamber and placed in an oven at 58°C with a rocker for 16-20 hours. After the hybridization, the BeadChips were washed and stained in a series of washes and stains as outlined in the Illumina protocol. The BeadChips were scanned on the Illumina Iscan.
Hybridization protocol
Standard Illumina hybridization protocol
Scan protocol
Standard Illumina scanning protocol
Description
Asymptomatic
Data processing
The data were normalised using quantile normalisation with lumi (lumi_2.4.0) in R version 2.13.2 (2011-09-30)
