|
| Status |
Public on Feb 01, 2015 |
| Title |
atria MR1, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Sus scrofa atria, mitral regurgitation pigs untreated with valsartan, sacrificed 12 months after surgery
|
| Organism |
Sus scrofa |
| Characteristics |
tissue: left atria
|
| Treatment protocol |
Mitral regurgitation was created in Lanyu miniature pigs by a 2.8 mm hole on the middle scallop of the posterior mitral leaflet in the MR and MRV groups. Pigs in the MRV groups additionally received valsartan (3.43 mg/kg/day), a type I angiotensin II receptor blocker, from one week before surgery and then daily after surgery.
|
| Growth protocol |
Pigs in the MR, MRV and NC groups received usual diet and were raised in the cages under the temperature of 20-24 ℃ and humidity of 50-70%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
see standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45℃ on GeneChip Procine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 7G.
|
| Description |
Expression data from Sus scrofa atria aged 18 months
|
| Data processing |
The CEL files were generated using Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Signal intensities were derived from CEL files and Signal intensity derived from the CEL files, using RMA method
|
| |
|
| Submission date |
Dec 20, 2012 |
| Last update date |
Feb 01, 2015 |
| Contact name |
Tzu-Hao Chang |
| Organization name |
Taipei Medical University
|
| Department |
Graduate Institute of Biomedical Informatics
|
| Street address |
250 Wu-Xin Street
|
| City |
Taipei |
| ZIP/Postal code |
110 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE43072 |
Expression data from Sus scrofa atria |
|
