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Sample GSM105379 Query DataSets for GSM105379
Status Public on Jul 28, 2006
Title retroperitoneal adipose tissue, Day 7, CLA treat
Sample type RNA
 
Source name mouse retroperitoneal fat pad
Organism Mus musculus
Characteristics JMin_MG4302_051905_9_D7CL
Genotype:C57BK/6J
Age: 9 wk old mice
Treatment protocol Two retroperitoneal fat pads excised from each of 10 mice, pooled, frozen in liquid N2 and later extracted in Trizol solution.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Genome 420 2.0 arrays using the Hybridization Oven 640, the hybridized array washed with the Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000
Description Gene expression data from retroperitoneal adipose tissue of C57BK/6J mice fed 0.5% trans-10 cis-12 conjugated linoleic acid.
Data processing The data were analyzed with robust multi-array analysis RMA. The RMA signal values wre log2 transformed.
 
Submission date Apr 13, 2006
Last update date Jul 28, 2006
Contact name Chris LaRosa
E-mail(s) plarosa1@unl.edu
Phone 4024724198
Fax 402472-3139
Organization name University of Nebraska, Lincoln
Department Center of Biotechnology
Street address 19th & Vine
City Lincoln
State/province NE
ZIP/Postal code 68588-0665
Country USA
 
Platform ID GPL1261
Series (1)
GSE4671 Microarray Analysis of the Delipidation of White Adipose Tissue of Mice Fed Conjugated Linoleic Acid

Data table header descriptions
ID_REF
VALUE RMA calculated signal intensity

Data table
ID_REF VALUE
1415670_at 8.62
1415671_at 9.65
1415672_at 9.9
1415673_at 8.05
1415674_a_at 8.66
1415675_at 7.55
1415676_a_at 10.83
1415677_at 8.34
1415678_at 9.66
1415679_at 9.96
1415680_at 8.44
1415681_at 8.46
1415682_at 6.47
1415683_at 10.43
1415684_at 7.95
1415685_at 7.96
1415686_at 9.19
1415687_a_at 12.8
1415688_at 9.01
1415689_s_at 5.92

Total number of rows: 45101

Table truncated, full table size 718 Kbytes.




Supplementary data files not provided

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