|
| Status |
Public on Sep 22, 2014 |
| Title |
5355441025_E |
| Sample type |
RNA |
| |
|
| Source name |
Unirradiated Control
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129S2 K-rasLA1
Sex: F
tissue: lung; left lobe
|
| Treatment protocol |
Animals were transported to Brookhaven National Laboratory and either left unirradiated or irradiated with a fractionated or acute dose of 1.0 GeV/nucleon 56Fe- particles.
|
| Growth protocol |
Animals were bred and housed in ventilated microisolator cages within a specific pathogen free facility at UT Southwestern Medical Center at Dallas.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole left lung of euthanized animals were flash frozen. RNA was extracted from frozen tissue using Qiagen Rneasy Plus Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
G12D mutant K-ras
|
| Data processing |
Data was processed using tools in Bioconductor/R. Quality control and outlier dection with lumi; background correction, quantile normalization, and log2 transformation with MBCB. One outlier (5355441026_D) was removed during quality control procedures.
|
| |
|
| Submission date |
Nov 13, 2012 |
| Last update date |
Sep 22, 2014 |
| Contact name |
Kimberly Batten |
| E-mail(s) |
[email protected]
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Cell biology
|
| Lab |
Shay/Wright
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
73509 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE42233 |
Expression profiling of murine lung 70 days following exposure to fractionated or acute dose of 1.0 Gy 56Fe- particle irradiation |
|
