|
| Status |
Public on Jul 01, 2006 |
| Title |
MDS12 |
| Sample type |
RNA |
| |
|
| Source name |
CD34+ cells isolated from the bone marrow of an MDS patient
|
| Organism |
Homo sapiens |
| Characteristics |
Subtype: RA
Karyotype: del(5q)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Two-cycle Affymetrix protocol from 50 nanograms total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation. 10 micrograms of cRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using a GeneChip Scanner 3000
|
| Description |
Gene expression data from CD34+ cells isolated from bone marrow
|
| Data processing |
Affymetrix CEL files were used for Robust MultiChip Analysis (RMA)
|
| |
|
| Submission date |
Apr 05, 2006 |
| Last update date |
Aug 22, 2006 |
| Contact name |
Andrea Pellagatti |
| E-mail(s) |
[email protected]
|
| Phone |
00441865222911
|
| Organization name |
University of Oxford
|
| Department |
NDCLS, RDM
|
| Lab |
LLR Molecular Haematology Unit
|
| Street address |
John Radcliffe Hospital
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE4619 |
Gene expression profiling of CD34+ cells from MDS patients and normal controls |
|
