|
| Status |
Public on Jun 01, 2013 |
| Title |
Heart 3 |
| Sample type |
RNA |
| |
|
| Source name |
Normal Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: Human Adult Normal Tissue
tissue: Heart
supplier: biochain
catalog number: R1234122-P
|
| Extracted molecule |
total RNA |
| Extraction protocol |
On average, 50 7 um-thick cryostat sections were prepared for the extraction of total RNA from tumor cell-rich areas, which were identified by a surgical pathologist (YY) using every 10th section stained by May-Giemsa. Careful attention was paid to the mi
|
| Label |
CY3
|
| Label protocol |
600 ng total RNA was reverse-transcribed using oligo-dT primers (Invitrogen) and SuperScript II reverse transcriptase (Invitrogen) in the presence of 100 mCi of [33P]dCTP (Amersham Bioscience, Piscataway, NJ) according to the instructions for the Gene600
|
| |
|
| Hybridization protocol |
600 ng labelled product was hybridized to slides usinge a NimbleGen Hybridization Kit (cat. no. 05 583 934 001) according to manufacturers protocol, then hybridized for 16 hours at 42 degrees.
|
| Scan protocol |
The arrays were then exposed for 2 hours to an Imaging Plate and scanned at 25-mm resolution with a BAS5000 phosphoimager (Fuji Photo Film), images of the hybridized arrays were processed with L Process (Fuji Photo Film) and signal intensities quantified
|
| Description |
cDNA was produced from total RNA using random primer labeling.
|
| Data processing |
The raw data was normalized using the Robust Microarray Average (RMA) procedure implemented via the oligo package in Bioconductor
|
| |
|
| Submission date |
Oct 31, 2012 |
| Last update date |
Jun 01, 2013 |
| Contact name |
Morten Muhlig Nielsen |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
Aarhus University
|
| Department |
Department of Molecular Medicine
|
| Street address |
Palle Juul-Jensens Bouleward 99
|
| City |
Aarhus |
| ZIP/Postal code |
DK-8200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL16228 |
| Series (1) |
| GSE41947 |
Tissue specific expression of non-coding RNAs |
|
