|
| Status |
Public on Oct 31, 2012 |
| Title |
HeLa treated with Cas-II-gly, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
HeLa cells treated with Cas-II-gly
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: cervical cancer (HeLa) cells
agent: Cas II-gly
|
| Treatment protocol |
Cells were treated with Cas II-gly in 6-well microplates by 6h. In order to diminish biological variability, pools of 3 wells were made and combined into a single sample before proceeding to chip hybridization.
|
| Growth protocol |
HeLa cells were mantained at room temperature in 5% CO2 under sterile conditions in DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated using AllPrep DNA-RNA Mini Kit 50.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to Affymetrix, Santa Clara CA Inc. protocol from 400 ng total RNA.
|
| |
|
| Hybridization protocol |
Hybridization to Affymetrix U133plus2 GeneChips, staining and washing were carried out as described (Affymetrix, Santa Clara).
|
| Scan protocol |
Scanning procedure was carried out as described by Affymetrix, Santa Clara.
|
| Description |
Gene expression data from HeLa cells treated with Cas-II-gly.
Cas_II-gly-1
|
| Data processing |
Data pre-processing was performed by using the affy library in BioConductor running under [R].
|
| |
|
| Submission date |
Oct 24, 2012 |
| Last update date |
Oct 31, 2012 |
| Contact name |
Karol Baca Lopez |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Genomic Medicine
|
| Street address |
Periferico sur
|
| City |
Mexico City |
| ZIP/Postal code |
14610 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE41827 |
Expression data from HeLa cells treated with Casiopeina Cas-II-gly |
|
