|
| Status |
Public on Apr 24, 2013 |
| Title |
Liver, Lactobacillus brevis119-2 containing diet administrated for 2 weeks, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, fed Lactobacillus brevis119-2 containing diet for 2 weeks
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: Male
strain: Sprague-Dawley
tissue: Liver
age: 7 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolatedusing Trizol reagent (Invitrogen Life Technologies Corp.), then purified with an RNeasy mini kit (Qiagen K.K., Tokyo, Japan).
|
| Label |
biotin
|
| Label protocol |
biotinylated aRNA was obtained from 100 ng of purified total RNA using a GeneChip 3’ IVT Express Kit (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
No additional information
|
| Data processing |
CEL files were quantified with the Factor Analysis for Robust Microarray Summarization (FARMS) method, using the statistical language R and Bioconductor.
|
| |
|
| Submission date |
Oct 22, 2012 |
| Last update date |
Apr 24, 2013 |
| Contact name |
Yuji Nakai |
| Organization name |
Hirosaki University
|
| Department |
Institute of Regional Innovation
|
| Lab |
Section of Food Sciences
|
| Street address |
2-1-1, Yanagawa
|
| City |
Aomori |
| State/province |
Aomori |
| ZIP/Postal code |
038-0012 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE41734 |
Effect of Lactobacillus brevis 119-2 isolated from turnip “Tsuda kabu” on hepatic cholesterol level in cholesterol-administrated rat |
|
