|
| Status |
Public on May 31, 2013 |
| Title |
PC-3_basalhTERT_ShortTelomeres_mockcre_2 |
| Sample type |
RNA |
| |
|
| Source name |
Human prostate cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: prostate cancer cell line PC-3
telomere status: Short
exogenous htert: absent
|
| Treatment protocol |
no treatment.
|
| Growth protocol |
1x10^6 cells were subcutaneously injected into five-week-old female nude mice with a BALB/c genetic background. When the tumor size reached at least 5 mm in diameter, mice were euthanized and tumor tissue was collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction from tumor xenografts were performed by using the RNeasy kit and Tissue Lyser (Qiagen) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
mock/cre+
|
| Data processing |
Gene-level expression signals were obtained by processing of raw data with the RMA procedure using GeneSpring GX (Agilent Technologies).
|
| |
|
| Submission date |
Oct 12, 2012 |
| Last update date |
May 31, 2013 |
| Contact name |
kyotaro HIRASHIMA |
| Organization name |
The JFCR-Cancer Inst.
|
| Department |
Cancer Chemotherapy Center
|
| Lab |
Division of Molecular Biotherapy
|
| Street address |
3-8-31 Ariake
|
| City |
Koto-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
135-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE41559 |
Expression data by telomere elongation in vivo (xenograft) |
|
