|
Status |
Public on Oct 12, 2012 |
Title |
SNAPC1_EGF_rep1 |
Sample type |
RNA |
|
|
Source name |
Hela
|
Organism |
Homo sapiens |
Characteristics |
cell line: Hela genotype/variation: SNAPC1_depleted treatment: EGF
|
Treatment protocol |
cells were starved in 1% serum overnight, then treated with 100 ng/microl of recombinant EGF
|
Growth protocol |
Hela cells were transfected with sh vectors and selected in puromycin, medium was DMEM + 10% FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
cells were harvested and lysed in RLT buffer (Qiagen), total RNA was extracted with Rneasy mini kit
|
Label |
biotin
|
Label protocol |
samples were biotin-labeled using the TargetAmp™-Nano Labeling Kit for Illumina Expression BeadChip (Epicentre)
|
|
|
Hybridization protocol |
Biotin labeled aRNA at 750ng was hybridized to Illumina HumanHT-12V4 expression Beadchip with Illumina HumanHT-12 v4 Expression BeadChip Kit(cat# BD-103-0204 ) Subsequent steps include washing, blocking, and streptavadin-Cy3 staining of the beadchip.
|
Scan protocol |
Fluorescence emission by Cy3 is quantitatively detected with Illumina BeadArray Reader( scan software : Illumina BeadSacn) and GenomeStudio software provides results in standard file format
|
Description |
SNAPC1_30_rep1 EGF_30min_rep1
|
Data processing |
Intensity raw data were log2 transformed and normalized by quantile normalization using BeadArray package on R
|
|
|
Submission date |
Oct 12, 2012 |
Last update date |
Oct 12, 2012 |
Contact name |
Alessandro Gardini |
E-mail(s) |
[email protected]
|
Phone |
305-243-7394
|
Organization name |
University of Miami
|
Department |
Sylvester Comprehensive Cancer Center
|
Lab |
Ramin Shiekhattar
|
Street address |
1501 NW 10 Avenue
|
City |
Miami |
State/province |
FL |
ZIP/Postal code |
33136 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE41528 |
Genome-wide analysis of the SNAPc complex [Illumina array] |
GSE41529 |
Genome-wide analysis of the SNAPc complex |
|