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Sample GSM100977 Query DataSets for GSM100977
Status Public on Apr 01, 2006
Title PND28 Rat Testis_Rat230-2_3hr MEHP_Animal#50_04005
Sample type RNA
 
Source name Sample Type: tissue, Tissue: testis
Organism Rattus norvegicus
Characteristics Array Type: Rat230_2
Amount to Core: 10
Units of Amount: ug
Ratio: 2.0
RNA Integrity Number: 9.8
Bioanalyzer Results: excellent
Strain or Line: Fischer
Individual: 50
Sacrifice Method: CO2
Age: 28
Units of Age: PND
Biomaterial provider Kamin Johnson, CIIT Centers for Health Research, 6 Davis Drive, RTP, NC, 27709, Stephanie Lahousse, [email protected], 919-558-1267
Treatment protocol in vivo Treatment: MEHP, Treatment Duration: 3, Units of Duration: hours, Treatment Time: 1:10, Units of Treatment Time: pm, Vehicle for Chemical: corn oil, Amount of Vehicle: 0.23, Route of Exposure: oral gavage, Units of Amount of Vehicle: ml, Dose or Concentration: 1, Units of Dose or Concentration: gm/kg bodyweight
Extracted molecule total RNA
Extraction protocol Extraction Method: Trizol
Additional Purification Method: Qiagen RNeasy Maxi Kit
Label biotin
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description KJ_1_3_50_Testis_MEHP_Rat230_2_04005: PND 28 Fischer rats were gavaged with a single dose of 1 gram/kg MEHP in corn oil or corn oil alone. The animals were sacrified 1, 2, 3, 6, or 12 hours after dosing. The testis were removed and RNA extracted using the QIAGEN RNeasy Maxi kit
Data processing Affymetrix GCOS 1.0
 
Submission date Mar 17, 2006
Last update date Mar 22, 2006
Contact name Kamin Johnson
E-mail(s) [email protected]
Phone 919-558-1439
Organization name CIIT Centers for Health Research
Department Biological Sciences
Street address 6 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL1355
Series (1)
GSE4514 Microarray analysis of MEHP gavaged PND28 male rats

Data table header descriptions
ID_REF Affymetrix probe name
VALUE GCOS-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 778.477 P 0.000195
AFFX-BioB-M_at 1312.85 P 4.4e-05
AFFX-BioB-3_at 806.965 P 4.4e-05
AFFX-BioC-5_at 1638.46 P 5.2e-05
AFFX-BioC-3_at 1583.12 P 4.4e-05
AFFX-BioDn-5_at 5534.77 P 4.4e-05
AFFX-BioDn-3_at 9235.07 P 4.4e-05
AFFX-CreX-5_at 18583.5 P 5.2e-05
AFFX-CreX-3_at 20159.7 P 4.4e-05
AFFX-LysX-3_at 21.7914 P 0.015175
AFFX-r2-Ec-bioB-5_at 962.244 P 0.000244
AFFX-r2-Ec-bioB-M_at 1223.94 P 0.000244
AFFX-r2-Ec-bioB-3_at 1072.69 P 0.000244
AFFX-r2-Ec-bioC-5_at 2151.51 P 0.000244
AFFX-r2-Ec-bioC-3_at 1889.43 P 0.000244
AFFX-r2-Ec-bioD-5_at 11938.2 P 0.000244
AFFX-r2-Ec-bioD-3_at 12509.1 P 0.000244
AFFX-r2-P1-cre-5_at 22124.8 P 0.000244
AFFX-r2-P1-cre-3_at 28231.6 P 0.000244
AFFX_Rat_GAPDH_5_at 7229.31 P 4.4e-05

Total number of rows: 31099

Table truncated, full table size 906 Kbytes.




Supplementary data files not provided

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