|
| Status |
Public on Sep 26, 2012 |
| Title |
MED1 overexpression #2 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP-MED1 overexpressed
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: prostate cancer cell
cell line: LNCaP
transfection: MED1 expression vector
|
| Treatment protocol |
Cells were transiently transfected with MED1 expression vector or empty vector control using Lipofectamine.
|
| Growth protocol |
LNCaP cells were cultured in RPMI-1640 containing 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNAeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChips. The arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix G7 GeneArray Scanner.
|
| Description |
Gene expression data from LNCap cells transfected with MED1 expression vector
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Sep 25, 2012 |
| Last update date |
Sep 26, 2012 |
| Contact name |
John Langenfeld |
| E-mail(s) |
[email protected]
|
| Phone |
(732)-235-7802
|
| Organization name |
Rutgers-RWJMS
|
| Department |
Surgery
|
| Lab |
MEB 536, P.O. Box 19
|
| Street address |
One Robert Wood Johnson Place
|
| City |
New Brunswick |
| State/province |
NJ |
| ZIP/Postal code |
08901 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE41150 |
Genes up and down regulated in LNCaP cells overexpressing MED1 |
|
