|
| Status |
Public on Jul 30, 2013 |
| Title |
WT mouse skin treated daily with TPA during 4 days and collected 24 hr later, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
WT mouse skin treated daily with TPA during 4 days and collected 24 hr later
|
| Organism |
Mus musculus |
| Characteristics |
strain background: FVB
genotype/variation: wild type
tissue: whole skin
|
| Treatment protocol |
Topic administration of TPA or acetone on the dorsal shaved skin of mice before extraction of skin.
WT mouse skin treated daily with TPA (phorbol ester 12-O-tetradecanoylphorbol-13 acetate, 6.8 nmol in 200 μl acetone) during 4 days and collected 24 hr later
|
| Growth protocol |
This experiment has been done with animals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was extracted from three independent exponential cultures of the appropriate cell lines using the RNAeasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Total RNA was quantified using 6000 Nano Chips (Agilent Technologies, Santa Clara, CA) and used (2.3 μg/sample) to generated labeled cRNA probes according to the manufacturer’s instructions (Affymetrix).
|
| |
|
| Hybridization protocol |
Mouse Gene 1.0 ST arrays (Affymetrix) under standard conditions
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
wt_tpa-4_2
|
| Data processing |
Upon microarray hybridization, the raw data was normalized with RMA using RMAexpress software (v.1.0.5).
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| |
|
| Submission date |
Sep 13, 2012 |
| Last update date |
Jul 30, 2013 |
| Contact name |
Ramon Garcia-Escudero |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
CIEMAT
|
| Department |
Biomedical Innovation
|
| Lab |
Molecular and Traslational Oncology
|
| Street address |
Avda Complutense 40, Ed 70A
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE40849 |
Vav proteins orchestrate a keratinocyte auto/paracrine program critical for skin tumorigenesis. |
|
