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| Status |
Public on Nov 01, 2017 |
| Title |
MyoD and FoxO3 mediated hotspot interaction orchestrates super-enhancer activity during myogenic differentiation (Foxo3 ChIP-seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Super-enhancers (SEs) are cis-regulatory elements enriching lineage specific key transcription factors (TFs) to form hotspots. A paucity of identification and functional dissection promoted us to investigate SEs during myoblast differentiation. ChIP-seq analysis of histone marks leads to the uncovering of SEs which remodel progressively during the course of differentiation. Further analyses of TF ChIP-seq enable the definition of SE hotspots co-bound by the master TF, MyoD and other TFs, among which we perform in-depth dissection for MyoD/FoxO3 interaction in driving the hotspots formation and SE activation.
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| Overall design |
Foxo3 ChIP-seq experiments performed in myoblast and myotube.
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| Contributor(s) |
Wang H, Sun H, Hu P, Peng XL, So KK, He L, Zhao Y, Zhou J |
| Citation(s) |
28575289 |
| |
| Submission date |
May 23, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Jiajian Zhou |
| E-mail(s) |
[email protected]
|
| Organization name |
Southern Medical University
|
| Department |
Dermatology Hospital
|
| Street address |
No. 2 Lujing Road, Yuexiu District,
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
NA |
| Country |
China |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA387570 |
| SRA |
SRP107865 |
