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| Status |
Public on Mar 07, 2018 |
| Title |
Epitope mapping of 5 monoclonal antibodies directed against meningococcal factor H binding protein by peptide microarray |
| Platform organism |
Neisseria meningitidis serogroup B |
| Sample organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
|
| Summary |
The aim of the study was to determine the epitope targeted by 5 different HumAbs and the cross-reactivity to linear peptide epitopes of 12 different factor H binding protein (fHbp) variants. The HumAbs were diluted at 1:200 and incubated on a custom PepStar Peptide Microarray platform printed with 363 different peptides.
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| Overall design |
A panel of 363 peptides (15-mer overlapping peptides with an offset of 4 amino acids) covering the entire sequence of 12 different fHbp variants (1.1, 1.4, 1.13, 1.14, 1.15, 2.16, 2.19, 2.25, 2.77, 3.28, 3.45, NL10A) were printed in triplicate on a custom PepStar Peptide Microarray. Process-control peptide (GGSGGGSDYKDDDDK) and human IgG were distributed in each array copy as controls. HumAbs were profiled for anti-human IgG specific analysis of reactivity to the peptide epitopes
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| Contributor(s) |
Bartolini E, Bruttini M |
| Citation(s) |
29487324, 31442074 |
| |
| Submission date |
May 12, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Erika Bartolini |
| Organization name |
GSK Vaccines
|
| Department |
AIMB
|
| Lab |
Molecular Biology
|
| Street address |
Via Fiorentina 1
|
| City |
Siena |
| State/province |
Italy |
| ZIP/Postal code |
53100 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL23475 |
JPT PepStar™ Peptide Microarrays [fHbp] |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE98883 |
Epitope mapping of human antibodies directed against 4CmenB protein components |
|
| Relations |
| BioProject |
PRJNA386496 |
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