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| Status |
Public on Mar 07, 2018 |
| Title |
Epitope mapping of human Fabs directed against 4CmenB protein components by protein microarray |
| Platform organism |
Neisseria meningitidis |
| Sample organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
|
| Summary |
The aim of the study was to determine the epitope targeted by a panel of Human Fabs. Fabs were diluted at 1:50 and incubated on a non-commercial Protein Microarray platform printed with fHbp, NHBA and NadA specific recombinant protein fragments and full length fHbp, NHBA and NadA of different variants.
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| Overall design |
A panel of recombinant fragments of different length spanning the entire sequence of fHbp, NHBA and NadA plus different full length fHbp, NHBA and NadA variants were printed in 8 or 16 replicated on non-commercial Protein Microarray. All fragments were expressed in E. coli as either glutathione S-transferase-, His-tagged or TRX-fusions, purified from the cytoplasmic fraction as soluble forms. Human IgG (at 8 different concentration points) was distributed in each array copy as control. Fabs were profiled for anti-Human IgG specific analysis of reactivity to the spotted proteins.
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| Contributor(s) |
Bartolini E, Bruttini M |
| Citation(s) |
29487324, 31442074 |
| |
| Submission date |
May 12, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Erika Bartolini |
| Organization name |
GSK Vaccines
|
| Department |
AIMB
|
| Lab |
Molecular Biology
|
| Street address |
Via Fiorentina 1
|
| City |
Siena |
| State/province |
Italy |
| ZIP/Postal code |
53100 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL23474 |
Protein Microarray_Neisseria meningitidis [fHbp, NHBA and NadA] |
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| Samples (37)
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| This SubSeries is part of SuperSeries: |
| GSE98883 |
Epitope mapping of human antibodies directed against 4CmenB protein components |
|
| Relations |
| BioProject |
PRJNA386495 |
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