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| Status |
Public on Jan 11, 2018 |
| Title |
Identification of trancripts regulated by the Listeria monocytogenes two-component system regulator VirR by RNA-seq |
| Organism |
Listeria monocytogenes |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here we use RNA sequencing to identify the regulon of the two-component system regulator VirR under normal growth conditions. We generated an in-frame deletion of the virR gene and extracted RNA from this strain grown to mid-log phase in LB as well as the 10403S parental strain. We then analyzed differential expression of transcripts between these two strains using RNA-seq
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| |
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| Overall design |
Differential gene expression analysis of the Listeria monocytogenes virR deletion mutant relative to parental 10403S strain
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| |
|
| Contributor(s) |
Grubaugh D, Higgins DE |
| Citation(s) |
29263107 |
| |
| Submission date |
May 01, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Daniel Grubaugh |
| E-mail(s) |
[email protected]
|
| Phone |
617-432-4157
|
| Organization name |
Harvard Medical School
|
| Department |
MBIB
|
| Lab |
Higgins
|
| Street address |
77 Ave Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21330 |
Illumina HiSeq 2500 (Listeria monocytogenes) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA384925 |
| SRA |
SRP105758 |
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