|
| Status |
Public on Mar 27, 2018 |
| Title |
Chemical Inhibition of Ezh2 Alters Ex Vivo B cell Differentiation and Gene Expression Dynamics |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand the role of Ezh2 in B cell differentiation B cells were stimulated ex vivo with LPS, Il2, and Il5 in the presence of DMSO or the selective Ezh2 inhibitor GSK343. Following 3 days culture, activated B cells and Plasmablasts were FACS isolated and RNA-seq was performed to identify the molecular effects of Ezh2 inhibition on B cell subsets during differentiation.
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| |
|
| Overall design |
RNAseq on ex vivo differentiated B cell subsets treated with GSK343 or DMSO
|
| |
|
| Contributor(s) |
Scharer CD, Boss JM |
| Citation(s) |
29703886 |
| |
| Submission date |
Apr 12, 2017 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Chris Scharer |
| E-mail(s) |
[email protected]
|
| Organization name |
Emory University
|
| Department |
Microbiology and Immunology
|
| Lab |
Chris Scharer
|
| Street address |
1510 Clifton Rd, Suite 3086A
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (15)
|
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| This SubSeries is part of SuperSeries: |
| GSE97698 |
Epigenetic regulation of in vivo B cell Differentiation |
|
| Relations |
| BioProject |
PRJNA382685 |
| SRA |
SRP103834 |
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