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| Status |
Public on Jul 17, 2017 |
| Title |
Tobacco mosaic virus infection alters phloem associated translatomes in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study we used vascular specific promoters and a translating ribosome affinity purification strategy to identify phloem-associated translatome responses to infection by tobacco mosaic virus (TMV) in the systemic host Arabidopsis thaliana ecotype Shahdara. Three different promoter:FLAG-RPL18 lines were used. These included two phloem specific promoters (pSUC2 and pSULTR2;2) as well as the more ubiquitously expressed cauliflower mosaic virus 35S promoter (p35S). Immunopurification of ribosome-mRNA complexes was accomplished by the method described in Reynoso et al. (Plant Functional Genomics: Methods and Protocols, 185-207; 2015). The dataset includes samples from the leaves of 5-week-old plants inoculated with TMV (1 mg/mL) or mock inoculated with sterile water.
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| |
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| Overall design |
12 samples, 2 conditions (TMV or mock infected), 3 promoter lines, 2 biological replicates
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| |
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| Contributor(s) |
Culver JN, Collum TD |
| Citation(s) |
28710959 |
| |
| Submission date |
Apr 11, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
James N Culver |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Maryland, College Park
|
| Department |
Plant Science and Landscape Architecture
|
| Street address |
4291 Fieldhouse Dr.
|
| City |
College Park |
| State/province |
MD |
| ZIP/Postal code |
20742 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19080 |
Illumina HiSeq 1500 (Arabidopsis thaliana) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA382580 |
| SRA |
SRP103816 |
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