 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 18, 2017 |
| Title |
Jak/Stat3 regulated global gene expression dynamics during late-stage reprogramming process |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed RNAseq analysis to dissect the dynamic transcriptome change during mouse iPSC induction, with or without blocking the Jak/Stat3 activity. We described Jak/Stat3 activity-specific global gene expression patterns, biological events, and regulation of key pluripotent genes, epigenetic modulators, and non-coding RNAs during the reprogramming process. We further demonstrated that Jak/Stat3 activity is essential for proper imprint of Dlk1-Dio3 region that is necessary for complete pluripotency establishment. Functional analysis revealed that one Jak/Stat3 downstream targets identified in our study - Esrrb significantly rescues reprogramming despite the inhibition of Jak/Stat3 activity. Our data illustrated novel mechanism in Jak/Stat3 promoted pluripotency establishment, which is valuable for further improvement of naïve-state iPSC generation across different species.
|
| |
|
| Overall design |
Examination of the RNA samples collected from reprogrammed cells at 18 days after retroviral OKSM transduction of OG-MEFs and cultured in reprogramming medium with LIF, with either Jaki or DMSO (control) treatment starting at day 3 (named hereafter Jaki-Stage 1 (S1) or DMSO-S1, respectively), or from iPSC colonies picked at day 21 and expanded one more passage (p2) (named hereafter Jaki-S2 or DMSO-S2, respectively)
|
| |
|
| Contributor(s) |
Wang L, Jiang Z, Huang D, Duan J, Huang C, Sullivan S, Vali K, Yin Y, Zhang M, Wegrzyn J, Tian X, Tang Y |
| Citation(s) |
29510661, 29212799 |
| |
| Submission date |
Mar 31, 2017 |
| Last update date |
Jul 08, 2019 |
| Contact name |
Xiuchun Tian |
| E-mail(s) |
[email protected]
|
| Organization name |
University of connecticut
|
| Department |
Animal Science
|
| Street address |
1390 Storrs Road, ABL220D
|
| City |
Storrs |
| State/province |
CT |
| ZIP/Postal code |
06269 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15907 |
AB 5500xl Genetic Analyzer (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA381142 |
| SRA |
SRP102805 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE97261_genes.fpkm_table_join_id.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...