|
| Status |
Public on Aug 10, 2018 |
| Title |
Proteus mirabilis MrpJ ChIP-seq |
| Organism |
Proteus mirabilis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Proteus mirabilis is a leading cause of catheter-associated urinary tract infections (UTIs) and urolithiasis. The transcriptional regulator MrpJ inversely modulates two critical aspects of P. mirabilis UTI progression: fimbria-mediated attachment to the urinary tract, and flagella-mediated motility. Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) was used for the first time in a CAUTI pathogen to probe for in vivo direct targets of MrpJ. ChIP-seq revealed 81 78 direct MrpJ targets, including genes for motility, fimbriae and a type VI secretion system (T6SS), and the putative MrpJ binding sequence ACnCnnnnnnnGnGT.
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| Overall design |
Four types of P. mirabilis samples were sequenced:1) empty vector with no ChIP antibody (control); 2) pMrpJ (produces His-tagged MrpJ) with no ChIP antibody (control); 3) empty vector with anti-His ChIP (control) and 4) pMrpJ with anti-His ChIP (experimental sample). Two biological replicates were sequenced for each sample type, for a total of 8 samples.
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| Contributor(s) |
Debnath I, Wade JT, Stringer AM, Pearson MM |
| Citation(s) |
30082479 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K22 AI083743 |
Contribution of Novel Frimbriae to Proteus Mirabilis Virulence |
NEW YORK UNIVERSITY SCHOOL OF MEDICINE |
MELANIE M. PEARSON |
| DP2 OD007188 |
Pervasive transcription in bacterial genomes |
WADSWORTH CENTER - HEALTH RESEARCH, INC. |
Joseph Thomas Wade |
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| |
| Submission date |
Mar 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Melanie M Pearson |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan Medical School
|
| Department |
Microbiology and Immunology
|
| Lab |
Melanie Pearson
|
| Street address |
1150 W Medical Center Dr
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-0620 |
| Country |
USA |
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| Platforms (1) |
| GPL23167 |
Illumina HiSeq 2500 (Proteus mirabilis) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA378651 |
| SRA |
SRP101644 |
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