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Status |
Public on Oct 11, 2019 |
Title |
Expression data from inducible ES stable cell line overexpressing specifically the human gene GTF2I |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
In order to identify the effects of the induction of the gene of interest (GTF2I) on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the inducible not-tagged cell line Transcriptome analysis of the inducible transgenic mouse ES cell line overexpressing the human isorofm of the gene GTF2I
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Overall design |
For the analysis on the GTF2I inducible cell line, total RNA was extracted from three biological replicates grown in medium deprived of Tetracycline for 24 hours; RNA extracted from un-induced clones was used as control.
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Contributor(s) |
De Cegli R |
Citation(s) |
31695049 |
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Submission date |
Feb 23, 2017 |
Last update date |
Jan 10, 2020 |
Contact name |
Rossella De Cegli |
E-mail(s) |
[email protected]
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Phone |
08119230692
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Organization name |
Tigem
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Street address |
Via Campi Flegrei 34
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City |
Pozzuoli |
ZIP/Postal code |
80078 |
Country |
Italy |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (6)
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GSM2501114 |
CTL, biological replicate 1 [hGTF2I_A1_T0] |
GSM2501115 |
CTL, biological replicate 2 [hGTF2I_B3_T0] |
GSM2501116 |
CTL, biological replicate 3 [hGTF2I_C6_T0] |
GSM2501117 |
GTF2I inducible clone, biological replicate1 [hGTF2I_A1_T24] |
GSM2501118 |
GTF2I inducible clone, biological replicate2 [hGTF2I_B3_T24] |
GSM2501119 |
GTF2I inducible clone, biological replicate3 [hGTF2I_C6_T24] |
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This SubSeries is part of SuperSeries: |
GSE96701 |
A transcriptomic study of Williams-Beuren syndrome associated genes in mouse embryonic stem |
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Relations |
BioProject |
PRJNA376512 |